Ratio derivative-zero crossing and successive derivative of ratio spectra for simultaneous determination of urea, creatinine, and uric acid in human urine samples

ABSTRACT. Two simple precise and accurate spectrophotometric techniques are developed for simultaneous determination of ternary mixture urea, creatinine, uric acid in humane urine sample. The first technique is a ratio derivative zero - crossing where measured urea at 216.61 nm, creatinine 260.56 283 nm. second successive spectra 203 261 287 respectively. These procedures do not need separation. proposed methods showed excellent linear range over the concentration ranges 1.0–15.0 μg/mL, 1.0–16.0 2.0–15.0 μg/mL acid, recoveries ranged from 97.10% to 101.9% 97.22% to102.70 % 97.45% to102.55 with relative standard deviations less than 1.56% 3.87% 3.71% acid. analytical eco-scale green procedure index tools were used evaluate how will affect environment. quantification human samples can be accomplished remarkable effectiveness by using suggested approaches.
 
 KEY WORDS: Urea, Creatinine, Uric Derivative spectrophotometry
 Bull. Chem. Soc. Ethiop. 2023, 37(4), 817-829. DOI: https://dx.doi.org/10.4314/bcse.v37i4.2